Welcome to Biotechr


Biotechr is written by Dr. Robert Kruse (@RobertLKruse), who holds a PhD and is currently completing his MD. His research work focused on infectious disease and immunology. This blog is focused on analyzing the latest developments in biotechnologies being developed in academia and industry, with a particular focus on biomedical therapeutics. I hope that the posts are interesting and useful, and hope you join in the discussion with guest posts on the site!

Disclaimer: The thoughts on this blog are not intended as any investment advice regarding any companies that might be discussed, and represent my opinion and not the opinions of my employer. This site is not designed to and does not provide medical advice, professional diagnosis, opinion, treatment or services to you or to any other individual.

Thursday, April 23, 2015

How low must HBsAg levels go?

by Robert Kruse


I wanted to write some commentary on HBsAg levels and the therapeutic goal for knockdown that must be achieved in order to have clinical responses. This is in response to some speculation from the current ILC 2015 meeting. I wanted to say as a disclaimer that I believe that siRNA and antisense drugs (Tekmira, Isis, Arrowhead, Analym) could very well work against HBV and mediate clinical response; I am just more apprehensive about the certainty of its clinical success. I also do not own any stock in the companies referenced here, and this is not meant to be investment advice, but a scientific discussion.

My fellow HBV commentator at the RNAi Therapeutics Blog suggests that if levels of HBsAg under 500 IU/mL are achieved, that a synergy between interferon and siRNA could be achieved and cure could happen. He suggested that a month treatment of siRNA could get this low level, and then interferon could finish the job.

Let's assume that theory is right. It makes me nervous that even in patients under this magical threshold, they only had 9% cure rate after an entire year of interferon treatment, which most patients can't even tolerate. Does that mean the siRNA combo plus interferon will only have a 9% cure rate? I would hope not, otherwise the therapy would not be very efficacious. Since siRNA knocks down all viral genes, there is the hope that other mechanisms could be at play that would increase this cure rate.  (edit: @RNAianalyst clarified reported data was for all patients with different HBsAg levels, so 9% cure rate was for combined group. In the link below though, HBeAg negative patients with HBsAg levels of ~200 IU/mL and pegasys and adefovir treatment had 17% cure rate (defined as HBsAg loss) at 2 years after 48 weeks of treatment. Definite improvement over non-responders with higher levels of HBsAg, but not still not a home run like HCV blockbuster medications)

I also want to comment on applying a retrospective clinical cohort analysis to prognostications about the potential of this therapy. The study by Dr. Joerg Petersen is likely true and is similar to other clinical reports I've read on pubmed before, that patients with baseline HBsAg that is lower will respond to treatment better. The problem is that if you compare a patient with 5000 IU/mL vs 500 IU/mL, there are likely a host of differences in their baseline status, namely different levels of ongoing immune responses and/or viral genotype.

The lower HBsAg patient possibly has a more vigorous ongoing polyclonal T cell response against HBV that is keeping the levels of HBsAg low - there must be a reason for the difference in the first place after all. It is likely then that adding interferon to this patient can help push that person's immune system over the top. In the example above, the HBeAg negative patients are negative for that antigen because of a more active immune response against HBV, which lent themselves for a 17% cure rate with IFN added versus HBeAg+ patients who had an 11% cure rate (differing levels of HBsAg modified this as well). Indeed, the type of immune response a person has greatly influences the effect of IFN on the course of HBV.

This situation is analogous to how checkpoint inhibitors for certain tumors are very efficacious, likely because the tumors are more immunogenic, so there is a better immune system at the site ready to fight the tumor. The checkpoint inhibitors can easily push the anti-tumor T cells over the top. On the other hand, the patient with the 5000 IU/mL baseline, even if artificially induced to have lower HBsAg levels to 500 IU/mL, still has a poor ongoing immune response that might not be boosted well by interferon. It's certainly possible that the HBsAg drop could help DC presentation and augment T cell polyclonality, but I don't know how long that might take and I'm not sure if anyone in the field does either. It's possible that their viral antigen specific T cells are already exhausted against the given epitopes and are beyond recovery. The siRNA is analogous to holding the target still, interferon could be bullets, but if the patient doesn't have a gun, ie effective adaptive immunity, it all might be a waste.

There is also the black box of how different viral genotypes secrete HBsAg at different levels, and different viral genotypes respond to interferon differently. This has been reported in several papers on pubmed, and I wonder how this might influence the goal biomarker levels of any siRNA treatment. I would guess in Dr. Peterson's previous study that the patients were all controlled for the same genotype (typically these studies are at centers in a certain area, so the geography dictates similar genotypes), but this is one confounder to look at in any future clinical reports.

In summary, I believe siRNA and ASO drugs are definitely exciting therapeutic modalities and potential game changers for HBV treatment, since they offer a new mechanism to fight the virus. I just want to add caution, though, to benchmarking certain data points and prognosticating success. HBV is one virus, but with a heterogeneous course across patients, the natural history of which is still being learned. The key immunological studies being discussed above have been limited since we can only really gain relevant data from chimpanzees and humans. It will be fascinating as an HBV researcher to see if knockdown could truly reactivate the immune system in all patients. Even if it doesn't in all, there could be a treatment cohort where a 100% cure rate is achieved, like the patients with better baseline status (<500IU/mL) discussed above. However this goes, it will at least be interesting from a basic science perspective.











43 comments:

  1. I find your blogs interesting and information.
    Even though sRNAi can be used to knock down all viral genes, it seems effort is concentrated on the s-antigen, for good reason. Replicor had demonstrated in several small human clinical trials with REP9AC that sustained svr, including loss of the s-antigen can be achieved in combination of with Interferon. The results have been confirmed with their recent HBV/HDV clinical trial with Rep2139ca.
    It would be interesting to investigate the effect of knock down of the e-antigen in HBeAg positive patients - will it lead to break down of immune tolerance and e-seroconversion?
    Finally, would like to hear your comments on Birinaapant, the 100% cure for HBV in animal models.

    ReplyDelete
  2. Thanks for your comment, Stephen. The effort is concentrated on HBsAg, because it is the most overexpressed protein that seems to act in a number of immunomodulatory roles. It's mRNA transcript levels are also the highest, and therefore a good target for siRNAs.

    The Replicor results do appear to be much more potent than siRNA's in knocking down HBsAg levels, and we will have a blog entry on Replicor later this week.

    You have an interesting idea on e-antigen. I wonder if siRNA treatment could make HBeAg seroconverstion to go much faster, even if HBsAg levels aren't eradicated. The data appears to show that HBeAg is readily knocked down by siRNAs but not sure if people have looked for that yet. HBeAg seroconversion is a good clinical outcome alone, so it could be worth something to Arrowhead, for example.

    For your last question, I think I will explore SMAC mimetics in a later blog post. I can only go off the data on Tetralogic's website, which appears to be good, but the HBV models they are using are unclear, so I don't want to speculate too much at this time.

    ReplyDelete
    Replies
    1. For Birinapant, you will find two papers published by the Walter Eliza Hall Institute team investigating its potential to treat HBV on behalf of Tetralogic in the recent editions of PNAS.
      Looking forward to reading your comments on REP2139 and Birinipant.

      Delete
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  3. Thanks for the tip on Tetralogic. I was literally on their site a few weeks ago and on pubmed and didn't see any publications yet. I'm looking over the paper right now.

    ReplyDelete
  4. BTW, Tetralogic had just "temporarily halted enrollment" of its clinical trial in Melbourne clinical trial.

    ReplyDelete
    Replies


    1. i have heard of him before, but after watching his documentary “healing spell” I was turned on to the full story

      about Dr atinga. He is a man who has the Cure for AIDS,Cancer, Sickle Cell, Herpes,warts,HPV,ALS Diabetes and every kind of

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      Delete
  5. Thanks for the tip. I'm really surprised by this, considering SMAC mimetics have been tested in other Phase I trials before. I understand theirs might be a different class though. The cranial nerve palsy thing is just kind of bizarre to me, not even liver related.

    ReplyDelete
  6. Bell's Palsy was known and reversible from their cancer clinical trials. Not much information about the present situation.

    ReplyDelete


  7. i have heard of him before, but after watching his documentary “healing spell” I was turned on to the full story

    about Dr atinga. He is a man who has the Cure for AIDS,Cancer, Sickle Cell, Herpes,warts,HPV,ALS Diabetes and every kind of

    illness, through a whole foods vegetarian diet and natural herbs, what he calls Electric Foods. He went to herbal school college,

    and has thousands of testimonials of healing people with Herpes, HIV AIDS, and HPV several other

    illnesses. Lisa “Left Eye” Lopes of TLC was cured of Herpes by him and she was so moved by his knowledge that she went on

    to spread the word about him in every way she could. She also opened a Cultural/Healing Center for Children in USA

    before she passed away tragically. This all inspired me that i had to contact Dr atinga, because i was having Herpes

    which could not be cure but was only managed, when i contacted Dr. atinga, he sent me some herbs which i took, and now i can tell you, i have be totally cured of Herpes, My Name is Joy wilson, I am from the South africa, and here is my email address .............joywilson953@gmail.com. Please if you have any form

    of illness especially STD, HPV, Infertility,HIV etc. you can contact him immediately through his Email Address

    dr.atingaspellhome@gmail.com

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    ReplyDelete
  8. I am very much delighted to every viewers that is reading this, my names is Gloria Francis, living in Canada, i want to inform the general public of how i got cured of Hepatitis b, i was diagnosed of Hepatitis b since 7years ago, this disease started circulating all over my body, and i have also taken treatment from my doctor,few weeks back i came on the internet to see if i will be able to get any information as in curing this disease, on my search i saw various testimony of Sam, who was healed from Herpes and Regina who also healed from Hepatitis B by HEALTH LAB, without any hesitation, i contacted their email: labhealth2@gmail.com and they started the remedies for me and i started using the medicine, after the period of days given to my by the HEALTH LAB, i was completely cured no doubt was found on my body. So viewers why wasting your time living with this STD infection? you can contact them now they are able to cure any kinds of disease, Hepatitis B ALS, MND, Epilepsy, Leukemia, Asthma, Cancer, HIV, Herpes etc, and others, contact them via Email: labhealth2@gmail.com

    ReplyDelete

  9. My Name Hermela i am a 45years old lady, i am from Ethiopia , but living in United State, My Hepatitis B (HBV) disease appeared at the age of 38, with no hope of a cure from the hospitals i have went to, i thought my life have come to an end, until a friend of mine told me about MED LAB who treatments all manners of diseases including Hepatitis B disease, I contacted them via their emial: medlab36@gmail.com and purchased the HBV medicine. I received the medicine through DHL couriers and i immediately commenced usage as prescribed, i used the medicine witin the few days i was instructed i became negative of (HBV), all my symptoms including Abdominal pain, Nausea and vomiting, Loss of appetite, was no more, I am HBV free, contact MED LAB email medlab36@gmail.com

    ReplyDelete
  10. I have not met Dr. UWENBO but have heard of his excellent work on people's life. We contacted Dr. Uwenbo and he started his work on my life and right now i am cured. i was diagnosed with Herpes And HIV and after taking Dr. Uwenbo Herbal medicine i was cured. You can reach him dr.uwenboherbalhome@gmail.com OR dr.uwenboherbalhome@hotmail.com WhatsApp +2349052309005

    ReplyDelete
  11. I want to share this wonderful testimony to the people all over the world on how i was cured of Hepatitis b disease by MED LAB, I'm from Philippine but living in Canada, i was living with hepatitis b for the past 10 years, just last month as i was browsing on the internet about this deadly disease, i saw a testimony of someone testifying of how he was cured from chronic hepatitis b, by MED LAB and i decided to also email medlab36@gmail.com and explain my problem, and they responded to me, then they send the medicine to me through delivery service. i was given instructions on how to use the treatment for somedays days and after the days given to me, i went for Hepatitis b test which i did and to my greatest surprise i was confirmed negative. all thanks be to MED LAB and God almighty for curing me of this deadly disease after 10 years and if you know that you are in this same problem email MED LAB now via email medlab36@gmail.com

    ReplyDelete
  12. All thanks to God, i am now negative of HBsAG, Contact: medlab36@gmail.com

    ReplyDelete
  13. I was diagnosed with HepB 3 months ago and was devastated by the news. I have been ill and sore for over 10 years, with no one being able to figure out what was wrong with me. It was always written off as a virus or infection. Then 2 years ago the severe muscle cramps started. I had them from scalp to toes, and it was when they ran the full spectrum of tests to figure the cramps out, that they discovered the HepB along with severe Vitamin D deficiency, which I link to the HepB. I was so tired and nauseous and in constant pain. I am just sick and tired of feeling sick and tired!!nothing was really working to help my condition.I went off the Sebivo (with the doctor’s knowledge) and started on hepatitis B herbal formula i ordered from Health Herbal Clinic, my symptoms totally declined over a 5 weeks use of the hepatitis B virus natural herbal formula the disease is totally reversed!! Visit there website www. healthherbalclinic. net

    ReplyDelete
  14. I was diagnose of hep B for more than 2 years, all the medical doctors said to me there is no cure but it can only be control. Ever since then i have been running heather scepter depressed looking for a solution to my problem, i ran into this online testimony of Mrs Stacey who have been cured of Hep B by this medical center she mention, i immediately contacted them, to God be thy glory i also got my cure am so happy today, please my advice is, if you have been living with Hep B contact them now for any kind of disease,don't waste time and get cured here is also their email healthmedlab@gmail.com

    ReplyDelete
  15. As a sign of gratitude for how my husband was saved from hepatitis b, i decided to reach out to those still suffering from this.
    My husband was diagnosed of hepatitis b in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he always have Joint pain , and he always complain of Weakness of the body . we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to hepatitis b. I never imagined hepatitis b has a natura cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. hepatitis b has a cure and it is a herbal cure contact the doctor for more info on drwilliams098675@gmail.com on how to get the medication. Thanks for reading my story

    ReplyDelete
  16. ALL THANKS TO DR WILLIAMS
    I was diagnosed of hepatitis B in 2011, I have tried all possible means to get cure but all my effort proved abortive, until a friend of mine introduced me to a herbal doctor , who prepare herbal medicine to cure different kind of diseases including hepatitis b virus (HBV), when i contacted this herbal doctor via his email, he sent me hepatitis b herbal medicine via courier service, when i received the herbal medicine he gave me step by step instructions on how to apply it, when i applied it as instructed i was totally cured from this virus within 8 days of usage. any body with similar problem can Contact this great herbal doctor via his email drwilliams098675@gmail.com for advice and for his product .and thanks to you admin for such an informative blog.

    ReplyDelete
  17. Am here to appreciate MED LAB for curing my hepatitis b virus. since 5 years now I have been living with this virus and it has been giving me challenges, I was so perplexed cause i have been taking several drugs to be cured but all of my effort was in vain,one morning i was browsing through the internet then i saw several testimonies about MED LAB curing people from Herpes virus, Hepatitis b and others, and immediately i contacted MED LAB email: medlab36@gmail.com i told them about my troubles and they told me that i will be cured, gave me some instructions and which i rightly followed. so they sent medicine for me and i used it just 8 days and everything was like a dream to me and my Hepatitis b virus was totally gone, why don't you contact them today and be free from your diseases because life is so sweet and opportunities are await us. contact via email; medlab36@gmail.com

    ReplyDelete
  18. Dear FRIENDS,
    now i have a beautiful smiling face and so are which you all, after reading my testimony. My name is Nita, I am 31 years old,
    And live with my boyfriend in Belgium, but I am UK. I have been diagnosed with hepatitis b since 2012, which the Doctors told me this condition was forever. I know how you feel and what you are going through, I don't have a baby though. I just want to tell you that I am cured now,with the help of doctor Williams herbal medication, the last time I can remember having hepatitis b was October this year. It was a long and difficult process but I am so thankful to dr williams I don't have it anymore. Please know you can be cured too. Accept what happened and is happening to you, forgive, let the past go, love yourself and others unconditionally and be thankful. Everything happens for a reason. If you want to be in contact with dr williams and share your own testimony and thoughts feel free to e-mail him on; drwilliams098675@gmail.com for advice and for his product.

    ReplyDelete
  19. My husband left me with 3 Children and went to stay with his Mistress whom i felt use a spell on him, I felt like ending it all, i almost committed suicide because he left us with nothing, i was emotionally down. But all thanks goes to Baba Alika, who i met online on one faithful day, as I was searching for tips to get my husband back home, I came across several testimonies about this particular man. Some people testified that he brought their Ex lover back, some testified that he help them to get pregnant,cure herpes simplex virus,and other sickness, and some testified that he prayed to stop divorce. He is amazing, i also come across one particular testimony, it was about a woman called Angeline, she testified about how he brought back her Ex lover in less than 2 days, and at the end of her testimony she dropped his email (babaalikahealinghome@gmail.com) After reading all these testimonies, I decided to give it a try. I contacted him and explained my problem to him. In just 48 hours, my husband came back to me. We resolved our issues, and we are even happier than ever. Baba Alika you are a gifted man and thank you for everything you had done in my life. If you have a problem and you are looking for a real and genuine spell caster, Try him anytime, he is the answer to your problems. you can contact him on email: babaalikahealinghome@gmail.com. he will surely help you solve your problem.

    ReplyDelete
  20. My name is Carel Silva, living in Qatar, i suffered from chronic hepatitis B , I spent alot on drugs, i never thought that a day like this will come and i will be able to give up on drugs. i found MED LAB, contact on blog when someone talked about MED LAB so i contacted them via email: medlab36@gmail.com and made purchase of the hepatitis Vaccine, i received the Vaccine through DHL within 3 days, when i received the Vaccine i applied it as prescribed and i was totally cured of hepatitis b within few's days of usages, i went back to my doctor and he confirmed i was free curable cured from the disease, am so happy now living a normal life again thanks to MED LAB. Viewers contact MED LAB via email: medlab36@gmail.com or whatsapp +27619522614

    ReplyDelete
  21. I was diagnosed with HepB 3 months ago and was devastated by the news. I have been ill and sore for over 10 years, with no one being able to figure out what was wrong with me. It was always written off as a virus or infection. Then 2 years ago the severe muscle cramps started. I had them from scalp to toes, and it was when they ran the full spectrum of tests to figure the cramps out, that they discovered the HepB along with severe Vitamin D deficiency, which I link to the HepB. I was so tired and nauseous and in constant pain. I am just sick and tired of feeling sick and tired!!nothing was really working to help my condition.I went off the Sebivo (with the doctor’s knowledge) and started on hepatitis B herbal formula i ordered from Health Herbal Clinic, my symptoms totally declined over a 5 weeks use of the hepatitis B virus natural herbal formula the disease is totally reversed!! Visit there website www. healthherbalclinic. net

    ReplyDelete
  22. What a great and inspiring story! I was married for seven years without any child, because of this my husband start acting strange, coming home lately and not spending time with me any more and he divorced me. So i became very sad and lost in life because my doctor told me there is no way for me to get pregnant this really make life miserable for me. until i came across a friend who told me about Baba Alika from the Internet, how he has helped a lot of women with this similar problems that i am going through so i contacted him on: (babaalikahealinghome@yahoo.com) and explained to him. he told me all what to provide before he could cast a reunite spell to bring back my husband which i did and he sent a powerful prayer which i had to say at mid night while he cast the love spell. It was a miracle, 24 hours later my one and only husband came back to me and apologize for all he has done and told me he is fully ready to support me in any thing i want,i quickly call Baba Alika and told him what was going on at that moment, and he also prepared and sent me a herbal medicine which he said it will cure every unwanted diseases or infections that has been stopping me from conceiving then instructed me on how to use it before meeting with my husband. Behold after using this native herbs and root, few weeks later i started feeling pregnancy signs all over me, truly i was pregnant i just gave birth to twins (boys) on the 1st of this month. I Vow to let the whole world know about Baba Alika for saving my relationship and for also giving me children i can proudly call my own today. For any woman who think it impossible, here is an opportunity for you to smile and bring happiness to your family kindly contact Baba Alika via Email on: (babaalikahealinghome@yahoo.com). Indeed, he is a God sent to bring back lost lovers and bring you happiness...

    ReplyDelete
  23. SAY NO TO AUTISM. I'm so happy today because of the wounder Dr Biodun has done with his autism herbal remedy in curing my 4 years old autistic son, it was hell for me to take good care of him because he always irritates me and i wished i never had him as a child back then he always wee on his body, eyes not straight, deaf so much more i was terrified and put all my hope in God because i have used so many hospital medications but to no avail. until this day when i was online in search of autism treatment for kids, there was a testimony of a woman testifying on how her autistic daughter was cured with the herbs of Dr Biodun i quickly massage the email address she dropped in the testimony (biodunherbal@yahoo.com) and i got a reply from Dr Biodun then i explained to him and he sent me his autism herbal medicine and gave me instructions on how to apply it on my son which i did as he commanded. Behold i watch my son changing gradually day by day while using the herbal cure on him, his voice became clearer, began to see and identify colours, making sentences and a lot of changes occurred, and i never believed that one day my son would be healed from autism embarrassment. contact him today don't be left out there is no harm in trying, believe me or not autism has a cure it is real am a living witness.

    ReplyDelete


  24. I am from New York. I was in trouble when doctor told me that I have been diagnosed with Genital Herpes… I though about my Family, I know my Family will face a serious problem when I’m gone, I lost hope and I wept all day, but one day I was surfing the internet I found Dr. ODUMA email. I emailed him and he guided me. I asked him for solutions and he started the remedies for my health. Thank God, now everything is fine, I’m cured by Dr. ODUMA herbal medicine, I’m very thankful to Dr. ODUMA and very happy with my hubby and family. email him on odumaspelltemple0@gmail.com or whatsapp Tel- +2347055176615 DOCTOR IDAHOSA CAN AS WELL CURE THE FOLLOWING DISEASE:- 1. HIV/AIDS 2. HERPES 3. CANCER 4. ALS 5. Arthritis 6. Shingles

    ReplyDelete
  25. With Dr Williams herb i was completely free from hepatitis b.You are an amazing doctor.Matching diagnosises aside, I just want to be your friend. Lol You inspire me on a feminist level. On an intelligence level. you are a good man doing so much for mankind, level.Thank you for being you.For keeping a lot of us . For reminding us that we aren’t a fucking statistic or a status, we are still PEOPLE. With brains and personalities and jobs and lives. I’ve been diagnosed for so many years now with hepatitis b, and the roller coaster of emotions is all too real.I’m sure I speak for everyone here when I say thank you. From the deepest part of my soul. THANK YOU.You give us all an indescribable level of comfort and absurdness in sharing our diagnoses and, almost a green light to continue to celebrate our lives,for you always been there.For every jerk who throws you hate, there are hundreds of us thanking the universe for you.for more information about Dr Williams product to hepatitis b
    you can email him on drwillliams098675@gmail.com

    ReplyDelete
  26. Thanks to Dr Williams I am so happy today, I have been suffering from hepatitis b for the past 8 years now, and i have spent a lot on western drugs which has all proved abortive, i have tried all means in life to become hepatitis b free , but there was no answer until i decided to try herbal solution and i found Dr Williams online and i contacted him and after I took his medication as instructed, i am now completely free from hepatitis b within those week of usage, i am so much happy, thanks to Dr Williams for helping me get my life back again without any form of crisis, i promise to tell your name and good deeds to the whole world,if you have hepatitis b you can email him on drwilliams098675@gmail.com for more information .

    ReplyDelete
  27. As a sign of gratitude for how my husband was saved from hepatitis b, i decided to reach out to those still suffering from this.
    My husband was diagnosed of hepatitis b in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he always have Joint pain , and he always complain of Weakness of the body . we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to hepatitis b. I never imagined hepatitis b has a natural cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. hepatitis b has a cure and it is a herbal cure contact the doctor for more info on drwilliams098675@gmail.com on how to get the medication. Thanks for reading my testimony.

    ReplyDelete
  28. DR WILLIAMS HERBAL CURE TO HEPATITIS B.
    I was always praying to have a testimony when ever i saw people sharing their own testimonies which really took me years but today i am the happiest father on earth.My son suffered hepatitis b for 5 years, after taken different kind of western medication without been cured.All these years my son was like a death person but today he is been cured.and you do not know how i feel having my son back to normal because it was not really easy for me having just two son and one been infected by this disease .I took time to search the internet which i found a lot of people thanking Dr. williams concerning the same problem and i discussed it with my wife and she agreed we should give a try so we order his product which my son took for 28 days and he is very much okay now.I will advise you to stop wasting time on all these western medicine because they only work for some times and the problem bounce back again.Contact Dr.at drwilliams098675@gmail.com for advice and for his product and be the next to give your testimony.

    ReplyDelete
  29. Hello Everybody,
    My name is Mrs Sharon Sim. I live in Singapore and i am a happy woman today? and i told my self that any lender that rescue my family from our poor situation, i will refer any person that is looking for loan to him, he gave me happiness to me and my family, i was in need of a loan of $250,000.00 to start my life all over as i am a single mother with 3 kids I met this honest and GOD fearing man loan lender that help me with a loan of $250,000.00 SG. Dollar, he is a GOD fearing man, if you are in need of loan and you will pay back the loan please contact him tell him that is Mrs Sharon, that refer you to him. contact Dr Purva Pius,via email:(urgentloan22@gmail.com) Thank you.

    ReplyDelete
  30. Hello Everybody,
    My name is Mrs Sharon Sim. I live in Singapore and i am a happy woman today? and i told my self that any lender that rescue my family from our poor situation, i will refer any person that is looking for loan to him, he gave me happiness to me and my family, i was in need of a loan of $250,000.00 to start my life all over as i am a single mother with 3 kids I met this honest and GOD fearing man loan lender that help me with a loan of $250,000.00 SG. Dollar, he is a GOD fearing man, if you are in need of loan and you will pay back the loan please contact him tell him that is Mrs Sharon, that refer you to him. contact Dr Purva Pius,via email:(urgentloan22@gmail.com) Thank you.

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  31. ALL THANKS TO DR WILLIAMS
    I was diagnosed of hepatitis B in 2011, I have tried all possible means to get cure but all my effort proved abortive, until a friend of mine introduced me to a herbal doctor , who prepare herbal medicine to cure different kind of diseases including hepatitis b virus (HBV), when i contacted this herbal doctor via his email, he sent me hepatitis b herbal medicine via courier service, when i received the herbal medicine he gave me step by step instructions on how to apply it, when i applied it as instructed i was totally cured from this disease within 1 months of usage. any body with similar problem can Contact this great herbal doctor via his email drwilliams098675@gmail.com for advice and for his product,and thanks to you admin for such an informative blog.

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  32. SPECIAL THANKS TO DR WILLIAMS FOR THE HEPATITIS B CURED HE RENDER TO ME.
    I'm giving a testimony about Dr. WILLIAMS the great Herbal man, he has
    the herbal cure to hepatitis b, he cured my hepatitis b disease,
    I went through different website I saw a testimonies about this
    Dr Williams, I was like: 'Dr Williams have the hepatitis b cure why are
    people still suffering from it?' I thought of it, then I contact Dr Williams, I didn't believe him that much, I just wanted to give him a try, he replied my mail and Needed some Information about me, then I sent them to him, he prepared the (CURE) and sent it to me through Courier Service he gave my details to the Courier Office, they told me that 1-3 days I will receive the package, and i took the medicine as prescribed by Dr Williams and I went for check-up 1 month after finishing the medicine, then the hepatitis b was not there anymore, if you are suffering from hepatitis b you can also contact him on his via email address: drwilliams098675@gmail.com for his help

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  33. [HOW I GOT CURED FROM GENITAL HERPES VIRUS )
    I was diagnosed with GENITAL HERPES VIRUS , my doctor told me it has no permanent cure, this virus affected me so badly that i was so ashamed of my self, this continued until a friend of mine Anna told me about Dr OLIHA from west Africa who cured her mother from GENITAL HERPES VIRUS, I contacted this herbal doctor and he sent me the herbal medicine through courier service, when i received it i applied it for 2 week with the instruction and i was totally cured from GENITAL HERPES VIRUS permanently within 7 to 8 days of usage. if you are passing through the same problem you can contact him via his email you should know about his natural herbal treatment, Dr OLIHA email is been attached to my post reach him for help. via email oliha.miraclemedicine@gmail.com add him on whatsapp line or call +2349038382931.
    he can also cured this disease,CANCER,ALS,HPV WARTS,HIGH BLOOD PRESSURE,STROKE.

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  34. I was diagnose April 10,2016 and find out I’m HIV positive.I was scared because there is no cure for HIV/AIDS but today some people still don’t believe that there is cure for HIV, it can only be cured through Africans root and herbs,and our doctor’s here in USA few of them know’s about the African herbal medicine can cure HIV but they chooses to hide it from us just to make a sales of ARV DRUGS. I did a research online finding way to get rid of my disease,I saw a comment about a herbal doctor on internet Name Dr IBUKUN ,who has cured several disease with his powerful herbal medicine, I contacted him on EMAIL (dribukunherbalhome@gmail.com) chat with him explain my self to him.He said he can cure hiv perfectly well , he gave me his request which i send to him. within 5 days he sent me the herbal medicine through UPS courier delivering service And told me how to take the medicine for 3 weeks to get cured,I did for 3 weeks, within this 3 weeks i notice a very big change in my health and i new some thing great has happened then i went to confirm my result it was absolutely negative.The doctor who new i was hiv positive was asking me how come i am negative, what did it took to get cure and were did i get this medicine from and how did i get rid of it I told him every thing about the herbal medicine that cure me. imagine doctor telling me not to let anyone know about it,I wasn’t shock though i knew they know about the herbal cure but chose to hide it in other to make sales on ARV DRUGS, *GENITAL & SIMPLEX HERPES… or need cure to diseases/viruses like.. *EPSTEIN-BARR VIRUS *PREDIABETES/DIABETES/DIABETES MELLITUS… *MENINGITIS… *MERS-CoV… *HIV.. *LEISHMANIASIS… *LOW SPERM COUNT…A DRY VAGINA *SHIGELLA INFECTION… *SYPHILLIS *CANCER… *LOWER RESPIRATORY INFECTION… *STAPHYLOCOCCUS AUREUS… *HEPATITIS… *SHINGLES… *YELLOW FEVER… *POLIO… *LUPUS… *STROKE… *FIBROID… *EPILEPSY… *TUBERCULOSIS… *LEUKAEMIA… *SICKLE CELL… *ASTHMA.. Etc.if you are HIV positive please contact my savior Email him (dribukunherbalhome@gmail.com)

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  35. i never believed that their could be any complete cure for fibroid with out going for surgery ,i saw people’s testimony on blog sites and Facebook page of how Dr Omohan prepare herbal cure for different king of disease,and with the help of his herb they where able to shrink there fibroid naturally. i had to try it too and you can,t believe that in just few weeks i started using it all my pains stop gradually . Right now i want to tell you all that i just give birth to a baby boy last month,and on thin now i have not had any pain, and i have just went for text last week and the doctor confirmed that there is no trace of any fibroid in my system . Glory be to God for leading me to this great Dr omohan I am so happy as i am sharing this testimony. My advice to you all who thinks that their is no herbal cure for fibroid that is Not true ,just contact him and get cure throughhis email: dromohanherbalmedicine@gmail.com or whatsapp +2348164816038

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  36. Wow a natural herb can cure epilepsy, I had epilepsy for over 14 years, i have spend a lot money getting drugs from the hospital to keep me healthy,i went to many hospitals for cure but there was no solution, One day I was in the office going through the internet when i saw a post about Dr Ofure herbal medicine on how he cure a woman with epilepsy, so i email him. He told me the things I need to do and also give me instructions to take, which I followed properly. Before I knew what is happening after two weeks To my greatest surprise after patiently going according to instruction given to me by Dr Ofure, my epilepsy was cure . I want to say a big thanks to Dr Ofure who God have sent to heal his people with his natural medicine i pray that God will bless you for the good work you are doing.for all who are still suffering from this disease, you can also contact him through his email on :drofureherbalhome@gmail.com. or message him on via whatsApp at +2348162868851 like they say his herbal remedy is the only permanent solution to Seizure

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  37. thanks to Dr omohan, who cured me of Fallopian tube blockage and fibroid. i married for over 5 years without a child of my own. because of the problem with my Fallopian tube and fibroid, until i came in contact with Dr omohan testimony and recommendations online, on how he has cured and healed so many people of the same problem and i decided to contact him,and explane my situation to him,few days later he sent me some herbal portion which i took for 14 days, and i became pregnant after taking dr omohan herbal medicine in three months time. in casse you want to reach him for a similar problem, you can contact him on his email: dromohanherbalmedicine@gmail.com for advice and for his product or whatasp him +2348164816038

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  38. I was searching for loan to sort out my bills& debts, then i saw comments about Blank ATM Credit Card that can be hacked to withdraw money from any ATM machines around you . I doubted thus but decided to give it a try by contacting {blankatm156@gmail.com} they responded with their guidelines on how the card works. I was assured that the card can withdraw $5,000 instant per day & was credited with $50,000 so i requested for one & paid the delivery fee to obtain the card, after 24 hours later, i was shock to see the UPS agent in my resident with a parcel{card} i signed and went back inside and confirmed the card work's after the agent left. This is no doubts because i have the card & has made used of the card. This hackers are USA based hackers set out to help people with financial freedom!! Contact these email if you wants to get rich with this Via: blankatm156@gmail.com

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  39. Dr. Iyabiye is a herbal specialist he treat/cure HEPATITIS and CIRRHOSIS and other deadly diseases. My name is Russel and I was ones a victim of chronic hepatitis B and liver cirrhosis, I got cured with his medication and I am safe and free now. I am testifying to his great work so that you too can be safe from hepatitis or any life threatening disease. His contacts are: iyabiyehealinghome@gmail.com Call/Whatsapp: +2348072229413

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  40. Having cancer is a Terrible Experience, my names are MARY JOHNSON I’m a citizen of United STATE,i was suffering of breast cancer and my family have taking me to all kind of hospital in US still yet no good result. I decided to go to the internet and search for cancer cure, so that was how I find a lady called ERIC PAULA she was testifies to the world about the goodness of a herbal man who has the root and herbs to cure all kind of disease and the herbal man email was there. So I decided to contact the herbal man for help to cure me from breast cancer. I contacted him and told him my problem he told me that I should not worry that my cancer will be cured, he told me that there is a medicine that he is going to give me that I will cook it and start drinking it for 2 weeks, so I ask how can I receive the cure that I am in US, he told me that I will pay for the delivery service. The courier service can transport it to me so he told me the amount I will pay, so my dad paid for the delivery fee. two days later I receive the cure from the courier service so i started using it as the herbal man instructed me to do before the week complete my cancer was healed and it was like a dream to me not knowing that it was physical I and my family were very happy about the miracle of Doctor so my dad wanted to pay him 2 million us dollars the herbal man did not accept the offer from my dad, but I don't know why he didn't accept the offer, he only say that I should tell the world about him and his miracle he perform so am now here to tell the world about him if you or your relative is having any kind of disease that you can't get from the hospital please contact drosohaberhome@gmail.com or call/whats-app +2348162084839 ....

    here is the dr website.
    https://sites.google.com/view/drosohaberhome/
    His blog page https://drosohaberhome.blogspot.com

    you can also email me for more info about DR oso via maryjohnson9700@gmail.com ALL THANK TO DR OSO .

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